Part:BBa_K801040:Design

SV40NLS-PhyB-linker-Gal4DBD (part for GAL4 based light switchable promoter system)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 550
  Illegal BamHI site found at 632
  Illegal XhoI site found at 583
  Illegal XhoI site found at 602
  Illegal XhoI site found at 2737
  Illegal XhoI site found at 3071
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2122
  Illegal BsaI site found at 2528
  Illegal BsaI site found at 2990
  Illegal SapI site found at 799
  

Composite part of BBa_K801030, BBa_K801030, BBa_K365005, BBa_K801035

Keywords: GAl4, PIF3, phytochrom B, light switchable
Abbreviations:

• UAS= upstream activity sequence

Design Notes

Related BioBrick:

• Related BioBricks:BBa_K801030: Polypeptide containing SV40 nuclear localization sequence, BBa_K801030: Polypeptide containing SV40 nuclear localization sequence, BBa_K365005: 20 aa linker RFC-25 , BBa_K801035: Linker-PIF3

Cloning details:

• Designed in RFC25

Quality control measures:

Backbone:

• Backbone name: pSB1C3

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

Organism:

References

Literature references:

Database references: